Browsing by Subject "PCR-DGGE"

Now showing items 1-3 of 3

    • Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet. 

      Grilli, Diego Javier; Fliegerová, Kateřina; Kopečný, Jan; Paez Lama, Sebastián; Egea, Vanina; Sohaefer, Noelia; Pereyra, Celia; Ruiz, María Soledad; Sosa, Miguel Angel; Arenas, Graciela Nora; Mrázek, Jakub (Comité editor Journal Anaerobe, 2016-07-12)
      High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. This study was therefore aimed to evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats to the high-grain diet (grain: hay e ratio of 40:60). The dietary transition of goats from the forage to the high-grain-diet resulted in the significant decrease of rumen fluid pH, which was however still higher than value established for acute or subacute ruminal acidosis was not diagnosed in studied ...

    • Caracterización de la población bacteriana ruminal de cabras Criollas bajo a diferentes condiciones de alimentación, utilizando diversas técnicas moleculares 

      Grilli, Diego; Arenas, Graciela Nora; Dayenoff, Patricio; Sohaefer, Noelia; Pereyra, Celia; Ruiz, Soledad; Quiroga, Luisa; Pereyra, Laura; Vernola, Santiago; Fliegerova, Katerina; Mrázek, Jakub (2016)
      Los recientes avances en técnicas de biología molecular permiten el análisis de bacterias anaerobias estrictas sin la necesidad de cultivarlas, identificando de esta manera muchas bacterias funcionales como nuevos objetivos de estudio. Nuestro equipo de investigación logró aislar y caracterizar a la especie bacteriana hemicelulolítica Pseudobutyrivibrio xylanivorans, a partir del rumen de cabras Criollas, proponiendo el uso de esta cepa como probiótico. Previamente a la introducción de bacterias probióticas es necesario conocer la diversidad y abundancia de las especies microbianas predominantes ...

    • Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage. 

      Grilli, Diego Javier; Mrázek, Jakub; Fliegerová, Kateřina; Kopečný, Jan; Paez Lama, Sebastian; Cerón Cucchi, María Esperanza; Sosa, Miguel Angel; Arenas, Graciela Nora (Editorial Livestock Science, 2016-08-02)
      Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and γ-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most ...